10779/crick.12639464.v1
Robert Edgington
Robert
Edgington
Katelyn M Spillane
Katelyn M
Spillane
George Papageorgiou
George
Papageorgiou
William Wray
William
Wray
Hitoshi Ishiwata
Hitoshi
Ishiwata
Mariana Labarca
Mariana
Labarca
Sergio Leal-Ortiz
Sergio
Leal-Ortiz
Gordon Reid
Gordon
Reid
Martin Webb
Martin
Webb
John Foord
John
Foord
Nicholas Melosh
Nicholas
Melosh
Andreas T Schaefer
Andreas T
Schaefer
Functionalisation of detonation nanodiamond for monodispersed, soluble DNA-nanodiamond conjugates using mixed silane bead-assisted sonication disintegration
The Francis Crick Institute
2020
Chemical Phenomena
DNA
Dynamic Light Scattering
Microscopy, Atomic Force
Nanodiamonds
Silanes
Solubility
Sonication
Schaefer FC001153
Tolar FC001185
PC
0601 Biochemistry and Cell Biology
0299 Other Physical Sciences
2020-07-15 11:08:54
Journal contribution
https://crick.figshare.com/articles/journal_contribution/Functionalisation_of_detonation_nanodiamond_for_monodispersed_soluble_DNA-nanodiamond_conjugates_using_mixed_silane_bead-assisted_sonication_disintegration/12639464
Nanodiamonds have many attractive properties that make them suitable for a range of biological applications, but their practical use has been limited because nanodiamond conjugates tend to aggregate in solution during or after functionalisation. Here we demonstrate the production of DNA-detonation nanodiamond (DNA-DND) conjugates with high dispersion and solubility using an ultrasonic, mixed-silanization chemistry protocol based on the in situ Bead-Assisted Sonication Disintegration (BASD) silanization method. We use two silanes to achieve these properties: (1) 3-(trihydroxysilyl)propyl methylphosphonate (THPMP); a negatively charged silane that imparts high zeta potential and solubility in solution; and (2) (3-aminopropyl)triethoxysilane (APTES); a commonly used functional silane that contributes an amino group for subsequent bioconjugation. We target these amino groups for covalent conjugation to thiolated, single-stranded DNA oligomers using the heterobifunctional crosslinker sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC). The resulting DNA-DND conjugates are the smallest reported to date, as determined by Dynamic Light Scattering (DLS) and Atomic Force Microscopy (AFM). The functionalisation method we describe is versatile and can be used to produce a wide variety of soluble DND-biomolecule conjugates.