EphrinB1/EphB3b coordinate bidirectional epithelial-mesenchymal interactions controlling liver morphogenesis and laterality
Jordi Cayuso
Aliaksandr Dzementsei
Johanna C Fischer
Gopal Karemore
Sara Caviglia
Josefin Bartholdson
Gavin J Wright
Elke A Ober
10779/crick.12619736.v1
https://crick.figshare.com/articles/journal_contribution/EphrinB1_EphB3b_coordinate_bidirectional_epithelial-mesenchymal_interactions_controlling_liver_morphogenesis_and_laterality/12619736
Positioning organs in the body often requires the movement of multiple tissues, yet the molecular and cellular mechanisms coordinating such movements are largely unknown. Here, we show that bidirectional signaling between EphrinB1 and EphB3b coordinates the movements of the hepatic endoderm and adjacent lateral plate mesoderm (LPM), resulting in asymmetric positioning of the zebrafish liver. EphrinB1 in hepatoblasts regulates directional migration and mediates interactions with the LPM, where EphB3b controls polarity and movement of the LPM. EphB3b in the LPM concomitantly repels hepatoblasts to move leftward into the liver bud. Cellular protrusions controlled by Eph/Ephrin signaling mediate hepatoblast motility and long-distance cell-cell contacts with the LPM beyond immediate tissue interfaces. Mechanistically, intracellular EphrinB1 domains mediate EphB3b-independent hepatoblast extension formation, while EpB3b interactions cause their destabilization. We propose that bidirectional short- and long-distance cell interactions between epithelial and mesenchyme-like tissues coordinate liver bud formation and laterality via cell repulsion.
2020-07-15 10:48:18
EphB3
EphrinB1
bidirectional
lateral plate mesoderm
liver
migration
morphogenesis
protrusion
repulsion
zebrafish
Animals
Body Patterning
Cell Movement
Cell Shape
Ephrin-B1
Ephrin-B3
Epithelium
Functional Laterality
Liver
Mesoderm
Morphogenesis
Pseudopodia
Receptors, Eph Family
Zebrafish
Zebrafish Proteins
Wilkinson, D FC001217
Elgar FC001075
BRF-ack
SB-ack
Developmental Biology
06 Biological Sciences
11 Medical and Health Sciences