10779/crick.12601775.v1 Stefan Boeing Stefan Boeing Laura Williamson Laura Williamson Vesela Encheva Vesela Encheva Ilaria Gori Ilaria Gori Rebecca E Saunders Rebecca E Saunders Rachael Instrell Rachael Instrell Ozan Aygün Ozan Aygün Marta Rodriguez-Martinez Marta Rodriguez-Martinez Juston C Weems Juston C Weems Gavin P Kelly Gavin P Kelly Joan W Conaway Joan W Conaway Ronald C Conaway Ronald C Conaway Aengus Stewart Aengus Stewart Michael Howell Michael Howell Ambrosius P Snijders Ambrosius P Snijders Jesper Q Svejstrup Jesper Q Svejstrup Multiomic analysis of the UV-induced DNA damage response The Francis Crick Institute 2020 Chromatin DNA Damage Databases, Factual HEK293 Cells Humans Internet Leupeptins Metabolic Networks and Pathways Nuclear Proteins Phosphorylation Protein-Serine-Threonine Kinases Proteome Proteomics RNA Polymerase II RNA, Small Interfering Transcription, Genetic Ubiquitination Ultraviolet Rays User-Computer Interface Svejstrup FC001166 CB PRT HTS CS-ack 0601 Biochemistry and Cell Biology 2020-07-15 08:54:23 Journal contribution https://crick.figshare.com/articles/journal_contribution/Multiomic_analysis_of_the_UV-induced_DNA_damage_response/12601775 In order to facilitate the identification of factors and pathways in the cellular response to UV-induced DNA damage, several descriptive proteomic screens and a functional genomics screen were performed in parallel. Numerous factors could be identified with high confidence when the screen results were superimposed and interpreted together, incorporating biological knowledge. A searchable database, bioLOGIC, which provides access to relevant information about a protein or process of interest, was established to host the results and facilitate data mining. Besides uncovering roles in the DNA damage response for numerous proteins and complexes, including Integrator, Cohesin, PHF3, ASC-1, SCAF4, SCAF8, and SCAF11, we uncovered a role for the poorly studied, melanoma-associated serine/threonine kinase 19 (STK19). Besides effectively uncovering relevant factors, the multiomic approach also provides a systems-wide overview of the diverse cellular processes connected to the transcription-related DNA damage response.