10779/crick.12601775.v1
Stefan Boeing
Stefan
Boeing
Laura Williamson
Laura
Williamson
Vesela Encheva
Vesela
Encheva
Ilaria Gori
Ilaria
Gori
Rebecca E Saunders
Rebecca E
Saunders
Rachael Instrell
Rachael
Instrell
Ozan Aygün
Ozan
Aygün
Marta Rodriguez-Martinez
Marta
Rodriguez-Martinez
Juston C Weems
Juston C
Weems
Gavin P Kelly
Gavin P
Kelly
Joan W Conaway
Joan W
Conaway
Ronald C Conaway
Ronald C
Conaway
Aengus Stewart
Aengus
Stewart
Michael Howell
Michael
Howell
Ambrosius P Snijders
Ambrosius P
Snijders
Jesper Q Svejstrup
Jesper Q
Svejstrup
Multiomic analysis of the UV-induced DNA damage response
The Francis Crick Institute
2020
Chromatin
DNA Damage
Databases, Factual
HEK293 Cells
Humans
Internet
Leupeptins
Metabolic Networks and Pathways
Nuclear Proteins
Phosphorylation
Protein-Serine-Threonine Kinases
Proteome
Proteomics
RNA Polymerase II
RNA, Small Interfering
Transcription, Genetic
Ubiquitination
Ultraviolet Rays
User-Computer Interface
Svejstrup FC001166
CB
PRT
HTS
CS-ack
0601 Biochemistry and Cell Biology
2020-07-15 08:54:23
Journal contribution
https://crick.figshare.com/articles/journal_contribution/Multiomic_analysis_of_the_UV-induced_DNA_damage_response/12601775
In order to facilitate the identification of factors and pathways in the cellular response to UV-induced DNA damage, several descriptive proteomic screens and a functional genomics screen were performed in parallel. Numerous factors could be identified with high confidence when the screen results were superimposed and interpreted together, incorporating biological knowledge. A searchable database, bioLOGIC, which provides access to relevant information about a protein or process of interest, was established to host the results and facilitate data mining. Besides uncovering roles in the DNA damage response for numerous proteins and complexes, including Integrator, Cohesin, PHF3, ASC-1, SCAF4, SCAF8, and SCAF11, we uncovered a role for the poorly studied, melanoma-associated serine/threonine kinase 19 (STK19). Besides effectively uncovering relevant factors, the multiomic approach also provides a systems-wide overview of the diverse cellular processes connected to the transcription-related DNA damage response.