Synthetic protein interactions reveal a functional map of the cell Lisa K Berry Guðjón Ólafsson Elena Ledesma-Fernández Peter H Thorpe 10779/crick.12594809.v1 https://crick.figshare.com/articles/journal_contribution/Synthetic_protein_interactions_reveal_a_functional_map_of_the_cell/12594809 To understand the function of eukaryotic cells, it is critical to understand the role of protein-protein interactions and protein localization. Currently, we do not know the importance of global protein localization nor do we understand to what extent the cell is permissive for new protein associations - a key requirement for the evolution of new protein functions. To answer this question, we fused every protein in the yeast Saccharomyces cerevisiae with a partner from each of the major cellular compartments and quantitatively assessed the effects upon growth. This analysis reveals that cells have a remarkable and unanticipated tolerance for forced protein associations, even if these associations lead to a proportion of the protein moving compartments within the cell. Furthermore, the interactions that do perturb growth provide a functional map of spatial protein regulation, identifying key regulatory complexes for the normal homeostasis of eukaryotic cells. 2020-07-01 17:08:17 GBP GFP S. cerevisiae cell biology localization nanobody Protein Binding Protein Interaction Maps Protein Transport Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Thorpe FC001183 0601 Biochemistry and Cell Biology