A reference genome and methylome for the Plasmodium knowlesi A1-H.1 line BenaventeErnest Diez de SessionsPaola Florez MoonRobert W GraingerMunira HolderAnthony A BlackmanMichael J RoperCally DrakeleyChristopher J PainArnab SutherlandColin J HibberdMartin L CampinoSusana ClarkTaane G 2020 Plasmodium knowlesi, a common parasite of macaques, is recognised as a significant cause of human malaria in Malaysia. The P. knowlesi A1H1 line has been adapted to continuous culture in human erythrocytes, successfully providing an in vitro model to study the parasite. We have assembled a reference genome for the PkA1-H.1 line using PacBio long read combined with Illumina short read sequence data. Compared with the H-strain reference, the new reference has improved genome coverage and a novel description of methylation sites. The PkA1-H.1 reference will enhance the capabilities of the in vitro model to improve the understanding of P. knowlesi infection in humans.