%0 Journal Article %A Bangani, Nonzwakazi %A Nakiwala, Justine %A Martineau, Adrian R %A Wilkinson, Robert J %A Wilkinson, Katalin A %A Lowe, David M %D 2020 %T HIV-1 infection impairs CD16 and CD35 mediated opsonophagocytosis of Mycobacterium tuberculosis by human neutrophils %U https://crick.figshare.com/articles/journal_contribution/HIV-1_infection_impairs_CD16_and_CD35_mediated_opsonophagocytosis_of_Mycobacterium_tuberculosis_by_human_neutrophils/12589361 %2 https://crick.figshare.com/ndownloader/files/23555981 %K CD4 Lymphocyte Count %K Flow Cytometry %K GPI-Linked Proteins %K HIV Infections %K Humans %K Immunologic Factors %K Mycobacterium tuberculosis %K Neutrophils %K Opsonin Proteins %K Phagocytosis %K Receptors, Complement 3b %K Receptors, IgG %K Tuberculosis %K Viral Load %K Wilkinson, R FC001218 %K 1103 Clinical Sciences %K Virology %X Using a flow cytometric assay, we investigated neutrophil-Mycobacterium tuberculosis opsonophagocytosis and the impact of HIV-1-infected serum on this process. The mean (±SD) percentage of neutrophils internalizing bacilli after 30 minutes incubation was significantly reduced by pretreatment with anti-CD16 (18.2% ± 8.1%, P < 0.001) or anti-CD35 antibody (23.2% ± 10.6%, P < 0.05) versus anti-CD4 controls (29.9% ± 8.1%). Blocking CD88 or CD11a did not affect internalization. Using heat-inactivated serum, maximal internalization was lower using HIV-1-infected serum versus HIV-1-uninfected. Using non-heat-inactivated serum, internalization decreased more rapidly with sequential dilutions of HIV-1-infected versus HIV-1-uninfected serum. CD16 and CD35 are important for neutrophil internalization of M. tuberculosis, whereas HIV-1 infection adversely affects opsonophagocytosis. %I The Francis Crick Institute