Novel initiator caspase reporters uncover previously unknown features of caspase-activating cells
Luis Alberto Baena-Lopez
Lewis Arthurton
Marcus Bischoff
Jean-Paul Vincent
Cyrille Alexandre
Reuben McGregor
10779/crick.12505406.v1
https://crick.figshare.com/articles/journal_contribution/Novel_initiator_caspase_reporters_uncover_previously_unknown_features_of_caspase-activating_cells/12505406
The caspase-mediated regulation of many cellular processes, including apoptosis, justifies the substantial interest in understanding all of the biological features of these enzymes. To complement functional assays, it is crucial to identify caspase-activating cells in live tissues. Our work describes novel initiator caspase reporters that, for the first time, provide direct information concerning the initial steps of the caspase activation cascade in Drosophila tissues. One of our caspase sensors capitalises on the rapid subcellular localisation change of a fluorescent marker to uncover novel cellular apoptotic events relating to the actin-mediated positioning of the nucleus before cell delamination. The other construct benefits from caspase-induced nuclear translocation of a QF transcription factor. This feature enables the genetic manipulation of caspase-activating cells and reveals the spatiotemporal patterns of initiator caspase activity. Collectively, our sensors offer experimental opportunities not available by using previous reporters and have proven useful to illuminate previously unknown aspects of caspase-dependent processes in apoptotic and non-apoptotic cellular scenarios.
2020-06-25 15:22:43
Apical caspase reporter
Apoptotic nuclear migration
Historical caspase reporter
Interkinetic nuclear migration
Non-apoptotic caspase functions
Animals
Apoptosis
Caspases
Cell Movement
Cell Nucleus
Cell Survival
Cell Tracking
Digestive System
Drosophila Proteins
Drosophila melanogaster
Enzyme Activation
Female
Genes, Reporter
Time Factors
Time-Lapse Imaging
Wings, Animal
Vincent FC001204
06 Biological Sciences
11 Medical and Health Sciences