%0 Journal Article %A Liu, Hon Wing %A Bouchoux, Céline %A Panarotto, Mélanie %A Kakui, Yasutaka %A Patel, Harshil %A Uhlmann, Frank %D 2020 %T Division of labor between PCNA loaders in DNA replication and sister chromatid cohesion establishment. %U https://crick.figshare.com/articles/journal_contribution/Division_of_labor_between_PCNA_loaders_in_DNA_replication_and_sister_chromatid_cohesion_establishment_/12465290 %2 https://crick.figshare.com/ndownloader/files/23086700 %K Chromosome Segregation %K Cohesion Establishment %K Ctf18 %K DNA Replication %K Eco1 %K PCNA %K Replication Factor C %K Rfc1 %K S. cerevisiae %K Sister Chromatid Cohesion %K AS-ack %K CB %K Uhlmann FC001198 %K 06 Biological Sciences %K 11 Medical and Health Sciences %K Developmental Biology %X Concomitant with DNA replication, the chromosomal cohesin complex establishes cohesion between newly replicated sister chromatids. Several replication-fork-associated "cohesion establishment factors," including the multifunctional Ctf18-RFC complex, aid this process in as yet unknown ways. Here, we show that Ctf18-RFC's role in sister chromatid cohesion correlates with PCNA loading but is separable from its role in the replication checkpoint. Ctf18-RFC loads PCNA with a slight preference for the leading strand, which is dispensable for DNA replication. Conversely, the canonical Rfc1-RFC complex preferentially loads PCNA onto the lagging strand, which is crucial for DNA replication but dispensable for sister chromatid cohesion. The downstream effector of Ctf18-RFC is cohesin acetylation, which we place toward a late step during replication maturation. Our results suggest that Ctf18-RFC enriches and balances PCNA levels at the replication fork, beyond the needs of DNA replication, to promote establishment of sister chromatid cohesion and possibly other post-replicative processes. %I The Francis Crick Institute