%0 Journal Article %A Lewis, Jacob S %A Costa, Alessandro %D 2020 %T Caught in the act: structural dynamics of replication origin activation and fork progression. %U https://crick.figshare.com/articles/journal_contribution/Caught_in_the_act_structural_dynamics_of_replication_origin_activation_and_fork_progression_/12301430 %2 https://crick.figshare.com/ndownloader/files/22676174 %K in silico reconstitution %K DNA replication %K biochemistry %K cryo-electron microscopy %K single molecule %K Costa FC001065 %K 0601 Biochemistry and Cell Biology %K 1101 Medical Biochemistry and Metabolomics %K Biochemistry & Molecular Biology %X This review discusses recent advances in single-particle cryo-EM and single-molecule approaches used to visualise eukaryotic DNA replication reactions reconstituted in vitro. We comment on the new challenges facing structural biologists, as they turn to describing the dynamic cascade of events that lead to replication origin activation and fork progression. %I The Francis Crick Institute