10779/crick.12301430.v1
Jacob S Lewis
Jacob S
Lewis
Alessandro Costa
Alessandro
Costa
Caught in the act: structural dynamics of replication origin activation and fork progression.
The Francis Crick Institute
2020
in silico reconstitution
DNA replication
biochemistry
cryo-electron microscopy
single molecule
Costa FC001065
0601 Biochemistry and Cell Biology
1101 Medical Biochemistry and Metabolomics
Biochemistry & Molecular Biology
2020-05-14 17:35:03
Journal contribution
https://crick.figshare.com/articles/journal_contribution/Caught_in_the_act_structural_dynamics_of_replication_origin_activation_and_fork_progression_/12301430
This review discusses recent advances in single-particle cryo-EM and single-molecule approaches used to visualise eukaryotic DNA replication reactions reconstituted in vitro. We comment on the new challenges facing structural biologists, as they turn to describing the dynamic cascade of events that lead to replication origin activation and fork progression.