10779/crick.12301430.v1 Jacob S Lewis Jacob S Lewis Alessandro Costa Alessandro Costa Caught in the act: structural dynamics of replication origin activation and fork progression. The Francis Crick Institute 2020 in silico reconstitution DNA replication biochemistry cryo-electron microscopy single molecule Costa FC001065 0601 Biochemistry and Cell Biology 1101 Medical Biochemistry and Metabolomics Biochemistry & Molecular Biology 2020-05-14 17:35:03 Journal contribution https://crick.figshare.com/articles/journal_contribution/Caught_in_the_act_structural_dynamics_of_replication_origin_activation_and_fork_progression_/12301430 This review discusses recent advances in single-particle cryo-EM and single-molecule approaches used to visualise eukaryotic DNA replication reactions reconstituted in vitro. We comment on the new challenges facing structural biologists, as they turn to describing the dynamic cascade of events that lead to replication origin activation and fork progression.