Role of spontaneous and sensory orexin network dynamics in rapid locomotion initiation
Mahesh M Karnani
Cornelia Schöne
Edward F Bracey
J Antonio González
Paulius Viskaitis
Han-Tao Li
Antoine Adamantidis
Denis Burdakov
10779/crick.12064152.v1
https://crick.figshare.com/articles/journal_contribution/Role_of_spontaneous_and_sensory_orexin_network_dynamics_in_rapid_locomotion_initiation/12064152
Appropriate motor control is critical for normal life, and requires hypothalamic hypocretin/orexin neurons (HONs). HONs are slowly regulated by nutrients, but also display rapid (subsecond) activity fluctuations in vivo. The necessity of these activity bursts for sensorimotor control and their roles in specific phases of movement are unknown. Here we show that temporally-restricted optosilencing of spontaneous or sensory-evoked HON bursts disrupts locomotion initiation, but does not affect ongoing locomotion. Conversely, HON optostimulation initiates locomotion with subsecond delays in a frequency-dependent manner. Using 2-photon volumetric imaging of activity of >300 HONs during sensory stimulation and self-initiated locomotion, we identify several locomotion-related HON subtypes, which distinctly predict the probability of imminent locomotion initiation, display distinct sensory responses, and are differentially modulated by food deprivation. By causally linking HON bursts to locomotion initiation, these findings reveal the sensorimotor importance of rapid spontaneous and evoked fluctuations in HON ensemble activity.
2020-04-17 11:17:14
Ensemble imaging
Hypothalamus
Locomotion
Sensorimotor processing
orexin/hypocretin neurons
Burdakov FC001055
1109 Neurosciences
1702 Cognitive Sciences
1701 Psychology
Neurology & Neurosurgery