10779/crick.11689356.v1 Giovanni Giuseppe Giobbe Giovanni Giuseppe Giobbe Claire Crowley Claire Crowley Camilla Luni Camilla Luni Sara Campinoti Sara Campinoti Moustafa Khedr Moustafa Khedr Kai Kretzschmar Kai Kretzschmar Martina Maria De Santis Martina Maria De Santis Elisa Zambaiti Elisa Zambaiti Federica Michielin Federica Michielin Laween Meran Laween Meran Qianjiang Hu Qianjiang Hu Gijs van Son Gijs van Son Luca Urbani Luca Urbani Anna Manfredi Anna Manfredi Monica Giomo Monica Giomo Simon Eaton Simon Eaton Davide Cacchiarelli Davide Cacchiarelli Vivian SW Li Vivian SW Li Hans Clevers Hans Clevers Paola Bonfanti Paola Bonfanti Nicola Elvassore Nicola Elvassore Paolo De Coppi Paolo De Coppi Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture. The Francis Crick Institute 2020 Li FC001105 Bonfanti - sec 2020-01-22 17:52:22 Journal contribution https://crick.figshare.com/articles/journal_contribution/Extracellular_matrix_hydrogel_derived_from_decellularized_tissues_enables_endodermal_organoid_culture_/11689356 Organoids have extensive therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, their clinical application is greatly limited by the lack of effective GMP-compliant systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix (ECM) hydrogels derived from decellularized tissues (DT) can provide an environment capable of directing cell growth. These gels possess the biochemical signature of tissue-specific ECM and have the potential for clinical translation. Gels from decellularized porcine small intestine (SI) mucosa/submucosa enable formation and growth of endoderm-derived human organoids, such as gastric, hepatic, pancreatic, and SI. ECM gels can be used as a tool for direct human organoid derivation, for cell growth with a stable transcriptomic signature, and for in vivo organoid delivery. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically.