10779/crick.11689356.v1
Giovanni Giuseppe Giobbe
Giovanni Giuseppe
Giobbe
Claire Crowley
Claire
Crowley
Camilla Luni
Camilla
Luni
Sara Campinoti
Sara
Campinoti
Moustafa Khedr
Moustafa
Khedr
Kai Kretzschmar
Kai
Kretzschmar
Martina Maria De Santis
Martina Maria
De Santis
Elisa Zambaiti
Elisa
Zambaiti
Federica Michielin
Federica
Michielin
Laween Meran
Laween
Meran
Qianjiang Hu
Qianjiang
Hu
Gijs van Son
Gijs
van Son
Luca Urbani
Luca
Urbani
Anna Manfredi
Anna
Manfredi
Monica Giomo
Monica
Giomo
Simon Eaton
Simon
Eaton
Davide Cacchiarelli
Davide
Cacchiarelli
Vivian SW Li
Vivian SW
Li
Hans Clevers
Hans
Clevers
Paola Bonfanti
Paola
Bonfanti
Nicola Elvassore
Nicola
Elvassore
Paolo De Coppi
Paolo
De Coppi
Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture.
The Francis Crick Institute
2020
Li FC001105
Bonfanti - sec
2020-01-22 17:52:22
Journal contribution
https://crick.figshare.com/articles/journal_contribution/Extracellular_matrix_hydrogel_derived_from_decellularized_tissues_enables_endodermal_organoid_culture_/11689356
Organoids have extensive therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, their clinical application is greatly limited by the lack of effective GMP-compliant systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix (ECM) hydrogels derived from decellularized tissues (DT) can provide an environment capable of directing cell growth. These gels possess the biochemical signature of tissue-specific ECM and have the potential for clinical translation. Gels from decellularized porcine small intestine (SI) mucosa/submucosa enable formation and growth of endoderm-derived human organoids, such as gastric, hepatic, pancreatic, and SI. ECM gels can be used as a tool for direct human organoid derivation, for cell growth with a stable transcriptomic signature, and for in vivo organoid delivery. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically.