10779/crick.11619690.v1 Adam M Thomas Adam M Thomas Sanja Brolih Sanja Brolih Joanna F McGouran Joanna F McGouran Afaf H El-Sagheer Afaf H El-Sagheer Denis Ptchelkine Denis Ptchelkine Morgan Jones Morgan Jones Neil Q McDonald Neil Q McDonald Peter J McHugh Peter J McHugh Tom Brown Tom Brown Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors. The Francis Crick Institute 2020 DNA Repair DNA-Binding Proteins Dimerization Endonucleases Humans Oligonucleotides Substrate Specificity Temperature McDonald FC001115 03 Chemical Sciences Organic Chemistry 2020-01-15 16:51:28 Journal contribution https://crick.figshare.com/articles/journal_contribution/Optimised_oligonucleotide_substrates_to_assay_XPF-ERCC1_nuclease_activity_for_the_discovery_of_DNA_repair_inhibitors_/11619690 We report the design and optimisation of novel oligonucleotide substrates for a sensitive fluorescence assay for high-throughput screening and functional studies of the DNA repair enzyme, XPF-ERCC1, with a view to accelerating inhibitor and drug discovery.