10779/crick.11619690.v1
Adam M Thomas
Adam M
Thomas
Sanja Brolih
Sanja
Brolih
Joanna F McGouran
Joanna F
McGouran
Afaf H El-Sagheer
Afaf H
El-Sagheer
Denis Ptchelkine
Denis
Ptchelkine
Morgan Jones
Morgan
Jones
Neil Q McDonald
Neil Q
McDonald
Peter J McHugh
Peter J
McHugh
Tom Brown
Tom
Brown
Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors.
The Francis Crick Institute
2020
DNA Repair
DNA-Binding Proteins
Dimerization
Endonucleases
Humans
Oligonucleotides
Substrate Specificity
Temperature
McDonald FC001115
03 Chemical Sciences
Organic Chemistry
2020-01-15 16:51:28
Journal contribution
https://crick.figshare.com/articles/journal_contribution/Optimised_oligonucleotide_substrates_to_assay_XPF-ERCC1_nuclease_activity_for_the_discovery_of_DNA_repair_inhibitors_/11619690
We report the design and optimisation of novel oligonucleotide substrates for a sensitive fluorescence assay for high-throughput screening and functional studies of the DNA repair enzyme, XPF-ERCC1, with a view to accelerating inhibitor and drug discovery.