A role for chromatin remodeling in cohesin loading onto chromosomes
Sofía Muñoz
Masashi Minamino
Corella S Casas-Delucchi
Harshil Patel
Frank Uhlmann
10779/crick.11382162.v1
https://crick.figshare.com/articles/journal_contribution/A_role_for_chromatin_remodeling_in_cohesin_loading_onto_chromosomes/11382162
Cohesin is a conserved, ring-shaped protein complex that topologically embraces DNA. Its central role in genome organization includes functions in sister chromatid cohesion, DNA repair, and transcriptional regulation. Cohesin loading onto chromosomes requires the Scc2-Scc4 cohesin loader, whose presence on chromatin in budding yeast depends on the RSC chromatin remodeling complex. Here we reveal a dual role of RSC in cohesin loading. RSC acts as a chromatin receptor that recruits Scc2-Scc4 by a direct protein interaction independent of chromatin remodeling. In addition, chromatin remodeling is required to generate a nucleosome-free region that is the substrate for cohesin loading. An engineered cohesin loading module can be created by fusing the Scc2 C terminus to RSC or to other chromatin remodelers, but not to unrelated DNA binding proteins. These observations demonstrate the importance of nucleosome-free DNA for cohesin loading and provide insight into how cohesin accesses DNA during its varied chromosomal activities.
2019-12-17 16:40:06
RSC
Saccharomyces cerevisiae
Scc2-Scc4
chromatin remodeling
cohesin
cohesin loader
sister chromatid cohesion
Cell Cycle Proteins
Chromatin Assembly and Disassembly
Chromosomal Proteins, Non-Histone
Chromosome Segregation
Chromosomes
DNA Repair
DNA-Binding Proteins
Nucleosomes
Saccharomyces cerevisiae Proteins
Sister Chromatid Exchange
Transcription, Genetic
Uhlmann FC001198
Diffley FC001066
CB
FERM-ack
HTS-ack
06 Biological Sciences
11 Medical and Health Sciences
Developmental Biology