γ-proteobacteria eject their polar flagella under nutrient depletion, retaining flagellar motor relic structures
Josie L Ferreira
Forson Z Gao
Florian M Rossmann
Andrea Nans
Susanne Brenzinger
Rohola Hosseini
Amanda Wilson
Ariane Briegel
Kai M Thormann
Peter B Rosenthal
Morgan Beeby
10779/crick.11359574.v1
https://crick.figshare.com/articles/journal_contribution/_-proteobacteria_eject_their_polar_flagella_under_nutrient_depletion_retaining_flagellar_motor_relic_structures/11359574
Bacteria switch only intermittently to motile planktonic lifestyles under favorable conditions. Under chronic nutrient deprivation, however, bacteria orchestrate a switch to stationary phase, conserving energy by altering metabolism and stopping motility. About two-thirds of bacteria use flagella to swim, but how bacteria deactivate this large molecular machine remains unclear. Here, we describe the previously unreported ejection of polar motors by γ-proteobacteria. We show that these bacteria eject their flagella at the base of the flagellar hook when nutrients are depleted, leaving a relic of a former flagellar motor in the outer membrane. Subtomogram averages of the full motor and relic reveal that this is an active process, as a plug protein appears in the relic, likely to prevent leakage across their outer membrane; furthermore, we show that ejection is triggered only under nutritional depletion and is independent of the filament as a possible mechanosensor. We show that filament ejection is a widespread phenomenon demonstrated by the appearance of relic structures in diverse γ-proteobacteria including Plesiomonas shigelloides, Vibrio cholerae, Vibrio fischeri, Shewanella putrefaciens, and Pseudomonas aeruginosa. While the molecular details remain to be determined, our results demonstrate a novel mechanism for bacteria to halt costly motility when nutrients become scarce.
2019-12-12 17:31:56
Flagella
Gammaproteobacteria
Plesiomonas
Pseudomonas aeruginosa
Shewanella putrefaciens
Vibrio cholerae
Rosenthal FC001143
SB-ack
06 Biological Sciences
11 Medical and Health Sciences
07 Agricultural and Veterinary Sciences
Developmental Biology